Effect of nicorandil on cardiomyocyte apoptosis in diabetic cardiomyopathy rats

doi:10.3877/cma.j.issn.2095-8757.2018.04.004

Abstract

Abstract:

Objective

To research the effect of nicorandil on cardiomyocyte apoptosis in diabetic cardiomyopathy (DCM) rats.

Methods

Thirty-two male Sprague-Dawley rats were randomly divided into control group A and DCM group B, nicorandil group C and glibenclamide group D. Group A was fed with conventional feed without intervention. Other groups were given measures inducing Insulin resistance by high-fat and high-calorie diet for 6 weeks, and the DCM model was established by intraperitoneal injection of streptozocin STZ (low dose streptozocin).After 2 weeks of continuous feeding with high fat and high sugar diet, the group B was given normal saline (4 mg/kg), group C was given nicorandil (10 mg/kg), meanwhile, group D was given glibenclamide (5 mg/kg) for 28 days. Then we anesthetized animals, collected the heart blood, after the specimens of the heart were taken after. Blood glucose and CRP were measured in each group, the expression of caspase-3, cleaved caspase-3 were detected by western blot method, TNF-α in plasma by Elisa.

Results

compared with group A, the blood glucose level in group B, C and D was significantly higher than that in group A (P<0.05), and there was no difference among group B, C and D (P>0.05), there was no significant difference in CRP among B, C and D group (P>0.05). Compared with group A, the expression of caspase-3、cleaved caspase-3 and TNF-α in group C and D were significantly higher than that in group A (P<0.05), there was no significantly difference in the expression between group C and A (P>0.05). Compared with group B, the expression in group C was significantly lower than that in group B (P<0.05). the expression in group D was not significantly higher than that in group B (P>0.05).

Conclusion

nicorandil can protect myocardium from apoptosis and has no effect on blood glucose metabolism.

Key words: Diabetic cardiomyopathy, Nicorandil, Apoptosis, Caspase3, TNF-α

Jing Yang, Ling Xi, Liting Yin, Donglin Wu. Effect of nicorandil on cardiomyocyte apoptosis in diabetic cardiomyopathy rats[J]. Chinese Journal of Geriatrics Research(Electronic Edition), 2018, 05(04): 15-19.

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References 20

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组别	只数	血糖（mmol/L, ±s）	CRP[mg/L, M (P25,P75)]
对照组	8	6.11±0.52	0.16(0.15,0.165)
DCM组	8	29.17±4.95	0.18(0.155,0.96)
尼可地尔组	8	32.60±0.67	0.145(0.135,0.625)
格列苯脲组	8	30.10±4.78	0.15(0.105,0.195)
检验值	?	F=109.538	χ²=3.998
P值	?	P<0.05	P>0.05

组别	只数	血糖（mmol/L, ±s）	CRP[mg/L, M (P25,P75)]
对照组	8	6.11±0.52	0.16(0.15,0.165)
DCM组	8	29.17±4.95	0.18(0.155,0.96)
尼可地尔组	8	32.60±0.67	0.145(0.135,0.625)
格列苯脲组	8	30.10±4.78	0.15(0.105,0.195)
检验值	?	F=109.538	χ²=3.998
P值	?	P<0.05	P>0.05

组别	只数	caspase-3/NAPDH	cleaved caspase-3/NAPDH	TNF-α(pg/ml)
对照组	8	0.138±0.004	0.0376±0.0011	12.3713±1.8171
DCM组	8	0.171±0.150	0.0481±0.0024	14.6575±0.6379
尼可地尔组	8	0.140±0.145	0.0387±0.0011	11.6800±1.0741
格列苯脲组	8	0.167±0.190	0.0418±0.0018	15.8138±0.7492
F值	?	21.029	90.222	36.918
P值	?	P<0.05	P<0.05	P<0.05

组别	只数	caspase-3/NAPDH	cleaved caspase-3/NAPDH	TNF-α(pg/ml)
对照组	8	0.138±0.004	0.0376±0.0011	12.3713±1.8171
DCM组	8	0.171±0.150	0.0481±0.0024	14.6575±0.6379
尼可地尔组	8	0.140±0.145	0.0387±0.0011	11.6800±1.0741
格列苯脲组	8	0.167±0.190	0.0418±0.0018	15.8138±0.7492
F值	?	21.029	90.222	36.918
P值	?	P<0.05	P<0.05	P<0.05

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