An analysis of serum human regenerating islet-derived 1α protein level in the early diagnosis of Alzheimer’s disease

doi:10.3877/cma.j.issn.2095-8757.2018.02.005

Abstract

Abstract:

Objective

This study was performed to investigate difference in the levels of serum regenerating islet-derived 1α protein (Reg-1α) expression in patients with mild cognitive impairments (MCIs), ADs and health controls, to explore the serum Reg-1α expression level as a biomarker for the early diagnosis of AD.

Methods

A total of 93 AD patients (AD group), 82 MCI patients (MCI group), and 110 healthy controls (normal group) aged 60 to 90 years who were admitted from June 2015 to June 2017 in Zhejiang Hospital were included. Peripheral blood samples of the subjects were tested for the expression levels of Reg-1α protein and mRNA in the serum by ELISA and real-time fluorescence quantitative RT-PCR, respectively.

Results

There were significant differences in the expression levels of Reg-1α protein and gene mRNA among the three groups (F=4.522, 2.629; all P<0.05). The levels of Reg-1α protein and gene mRNA in AD and MCI groups were significantly higher than those in the control group (q=-5.958, -6.914, -5.223, -7.679; all P<0.01). The level of Reg-1α protein in AD group was significantly higher than that in MCI group (q=7.681, P<0.01), but the expression level of Reg-1α mRNA was not significantly different from that in MCI group (q=3.028, P>0.05).

Conclusion

The expression level of reg-1 in serum was correlated with the progression of AD, and may be used as an early diagnostic marker of AD.

Key words: Regenerating islet-derived 1α protein, Cognitive Dysfunction, Alzheimer's disease, Diagnosis, Biomarkers

Xiaoli Liu, Miao Cai, Yan Li, Yu Jin, Shanhu Xu, Qinfen Shu. An analysis of serum human regenerating islet-derived 1α protein level in the early diagnosis of Alzheimer’s disease[J]. Chinese Journal of Geriatrics Research(Electronic Edition), 2018, 05(02): 27-31.

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References 19

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组别	例数	性别（男/女，例）	年龄（岁）	受教育年限（年）	吸烟史（例）	空腹血糖（mmol）
AD组	103	56/37	69.4±7.6	13.16±6.33	30	5.05±1.32
MCI组	82	45/37	67.2±6.1	13.67±4.12	37	4.82±1.55
正常组	110	61/49	66.2±7.4	15.14±6.54	39	4.89±1.12
χ²或F值	?	0.651	1.049	0.633	5.089	0.960
P值	?	>0.05	>0.05	>0.05	>0.05	>0.05

组别	例数	性别（男/女，例）	年龄（岁）	受教育年限（年）	吸烟史（例）	空腹血糖（mmol）
AD组	103	56/37	69.4±7.6	13.16±6.33	30	5.05±1.32
MCI组	82	45/37	67.2±6.1	13.67±4.12	37	4.82±1.55
正常组	110	61/49	66.2±7.4	15.14±6.54	39	4.89±1.12
χ²或F值	?	0.651	1.049	0.633	5.089	0.960
P值	?	>0.05	>0.05	>0.05	>0.05	>0.05

基因	引物名称	引物序列（5'-3'）
Reg-1α	正向引物	AGCATGGCTCAGACCA GCTCATAC
?	反向引物	CTAGTTTTTGAACTTGCAGAC
GAPDH	正向引物	GAGAGACCCTCACTGCTG
?	反向引物	GASTGGTAGATGACAAGGTGC

基因	引物名称	引物序列（5'-3'）
Reg-1α	正向引物	AGCATGGCTCAGACCA GCTCATAC
?	反向引物	CTAGTTTTTGAACTTGCAGAC
GAPDH	正向引物	GAGAGACCCTCACTGCTG
?	反向引物	GASTGGTAGATGACAAGGTGC

组别	例数	Reg-1α蛋白（pg/ml）	Reg-1αmRNA
AD组	103	2 383.89±73.45	1.94±0.27
MCI组	82	1 682.00±65.12	2.05±0.21
正常组	110	336.18±34.54	1.0±0.08
F值	?	4.522	2.629
P值	?	<0.01	<0.01

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