切换至 "中华医学电子期刊资源库"

中华老年病研究电子杂志 ›› 2019, Vol. 06 ›› Issue (01) : 21 -25. doi: 10.3877/cma.j.issn.2095-8757.2019.01.005

所属专题: 文献

基础研究

神经肌肉电刺激对糖尿病大鼠GLUT4基因表达的影响
夏婉1,(), 林坚1, 刘晓林1, 黄雄昂1, 李琳1, 汤洋1   
  1. 1. 310013 杭州,浙江医院康复医学科
  • 收稿日期:2018-10-16 出版日期:2019-02-28
  • 通信作者: 夏婉
  • 基金资助:
    浙江医药卫生科技项目(2016RCA001,2016142302); 浙江省卫生厅一般项目(Y2015100487)

The effects of neuromuscular electrical stimulation on the expression of GLUT4 mRNA in diabetic rats

Wan Xia1,(), Jian Lin1, Xiaolin Liu1, Xiong'ang Huang1, Lin Li1, Yang Tang1   

  1. 1. Rehabilitation Department, Zhejiang Hospital, Hanghzou 310013, China
  • Received:2018-10-16 Published:2019-02-28
  • Corresponding author: Wan Xia
  • About author:
    Corresponding author: Xia Wan, Email:
引用本文:

夏婉, 林坚, 刘晓林, 黄雄昂, 李琳, 汤洋. 神经肌肉电刺激对糖尿病大鼠GLUT4基因表达的影响[J/OL]. 中华老年病研究电子杂志, 2019, 06(01): 21-25.

Wan Xia, Jian Lin, Xiaolin Liu, Xiong'ang Huang, Lin Li, Yang Tang. The effects of neuromuscular electrical stimulation on the expression of GLUT4 mRNA in diabetic rats[J/OL]. Chinese Journal of Geriatrics Research(Electronic Edition), 2019, 06(01): 21-25.

目的

研究神经肌肉电刺激(neuromuscular electrical stimulation, NMES)对糖尿病大鼠股四头肌葡萄糖轻运载体4(glucose transporter 4, GLUT4)表达的影响。

方法

选取24只2型糖尿病大鼠,数字表法随机分为空白组、运动组、NMES组,每组各8只。运动组给予中等强度跑台训练干预;NMES组对大鼠双下肢股四头肌进行NMES。通过尾静脉采血测空腹血糖水平,采用酶联免疫吸附分析法检测大鼠胰岛素水平并计算胰岛素抵抗指数(insulin resistant index, IRI),采用RT-PCR法测定股四头肌GLUT4基因表达量。多组间的比较采用方差分析,进一步两组间的比较采用LSD-t检验。

结果

运动组和NMES组实验干预后血糖水平均显著下降(均P<0.01),空白组干预前后血糖水平无明显变化(均P>0.05)。3组大鼠实验干预前后胰岛素水平均无明显改变(t=1.023、2.283、1.775,均P>0.05);实验干预后运动组和NMES组IRI较实验干预前明显改善(t=10.216、6.748,均P<0.01)。实验干预前,3组大鼠空腹血糖水平及IRI无明显差异(F=1.138、1.040,均P>0.05);实验干预后,3组大鼠空腹血糖水平、IRI及GLUT4 mRNA表达水平的差异有统计学意义(F=38.415、9.976、275.123,均P<0.01)。实验干预后,运动组和NMES组血糖水平及IRI均显著低于空白组(均P<0.01),股四头肌GLUT4 mRNA的表达水平均显著高于空白组(均P<0.01);运动组与NMES组血糖水平及IRI的差异均无统计学意义(均P>0.05),运动组大鼠股四头肌GLUT4 mRNA的表达水平显著高于NMES组(P<0.01)。

结论

NMES训练可以降低糖尿病大鼠空腹血糖、改善胰岛素抵抗,其作用机制可能与骨骼肌GLUT4基因mRNA水平上调有关。

Objective

To study the effect of neuromuscular electrical stimulation (NMES) on the expression of glucose transporter 4 (GLUT4) of the quadriceps muscles in diabetic rats.

Methods

24 type 2 diabetic rats were randomly divided into control group, exercise group and NMES group. The exercise group trained on medium-strength treadmills. The NMES group received electrical stimulation of the quadriceps muscles of the lower limbs. Fasting blood glucose concentration was measured by tail vein blood, insulin concentration and insulin resistance index (IRI) were measured by ELISA, and GLUT4 gene expression was determined by RT-PCR. Anova was used for comparison among groups, and LSD-t test was used for further comparison among two groups.

Results

The blood glucose levels in the exercise group and NMES group were significantly decreased after intervention (P<0.01), and there was no significant change in control group (P>0.05). There was no significant change in insulin level before and after intervention in all groups (t=1.023, 2.283, 1.775, P>0.05), but the IRI was significantly decreased (t=10.216, 6.748, P<0.01) after intervention in the exercise group and NMES group. Before intervention, there was no significant difference in blood glucose level and IRI between the three groups (F=1.138, 1.040; P>0.05). After intervention, the statistical significance of blood glucose level, IRI and GLUT4 mRNA expression levels in the three groups were observed (F=38.415, 9.976, 275.123, P<0.01). After intervention, the blood glucose level and IRI in the exercise group and NMES group were significantly lower than those in control group (P<0.01), and the expression of GLUT4 mRNA in the quadriceps muscle was significantly higher than that in control group (P<0.01), but there was no significant difference in blood glucose level and IRI between the exercise group and NMES group (P>0.05), and the expression of GLUT4 mRNA in the quadriceps muscle of the exercise group was significantly higher than that in NMES group (P<0.01).

Conclusion

NMES training can improve insulin resistant to control blood sugar, related with the increase of the expression of GLUT4 mRNA.

表1 3组大鼠干预前后空腹血糖水平的变化(mmol/L,±s
表2 3组大鼠干预前后腹胰岛素水平和IRI的变化(ng/ml,±s
[1]
International Diabetes Federation. IDF Diabetes Atlas-7th Edition. Accesed on 1 feb 2016 at:

URL    
[2]
陈家伦.临床内分泌学[M].上海:上海科学技术出版社,2012:13-14.
[3]
Goodyear LJ, Kahn BB. Exercise, glucose transport, and insulin sensitivity[J]. Annu Rev Med, 1998, 49:235-261.
[4]
Rhodes CJ. Type 2 diabetes-a matter of beta-cell life and death[J]? Science, 2005, 307(5708):380-384.
[5]
柯俊,汤文璐.2006-2010年上海市口服抗糖尿病药物的不良反应报告分析[J].中国临床药学杂志,2011,20(6):351-356.
[6]
Herman MA, Kahn BB. Glucose transport and sensing in the maintenance of glucose homeostasis and metabolic harmony[J]. J Clin Invest, 2006, 116(7):1767-1775.
[7]
Rose AJ, Richter EA. Skeletal muscle glucose uptake during exercise: how is it regulated[J]? Physiology(Bethesda), 2005, 20:260-270.
[8]
Stringhini S, Tabak AG, Akbaraly TN, et al. Contribution of modifiable risk factors to social inequalities in type 2 diabetes: prospective White allⅡcohort study[J]. BMJ, 2012, 345:e5452.
[9]
Maffiuletti NA. Physiological and methodological considerations for the use of neur-omuscular electrical stimulation[J]. Eur J Appl Physiol, 2010, 110(2):223-234.
[10]
Joubert M, Metayer L, Prevost G, et al. Neuromuscular electrostimulation and insulin sensitivity in patients with type 2 diabetes: the ELECTRODIAB pilot study[J]. Acta Diabetol, 2015, 52(2):285-291.
[11]
Jabbour G, Belliveau L, Probizanski D, et al. Effect of low frequency neuromuscular electrical stimulation on glucose profile of persons with type 2 diabetes: a pilot study[j]. Diabetes Metab J, 2015, 39(3):264-267.
[12]
刘晓林,金肖青,俞李羚,等.神经肌肉电刺激治疗2型糖尿病效果观察[J].中国乡村医药,2017,24(5):25-26.
[13]
林强,吴毅,胡永善,等.电刺激对2型糖尿病大鼠骨骼肌细胞胰岛素信号通路的影响[J].中国康复医学杂志,2008,23(11):972-975.
[14]
Portha B. Programmed disorders of beta-cell development and function as one cause for type 2 diabetes? The GK rat paradigm[J]. Diabetes Metab Res Rev, 2005, 21(6):495-504.
[15]
Bedford TG, Tipton CM, Wilson NC, et al. Maximum oxygen consumption of rats and its changes with various experimental procedures[J]. J Appl Physiol Respir Environ Exerc Physiol, 1979, 47(6):1278-1283.
[16]
龚云,张鑫.不同强度跑台对大鼠2型糖尿病及胰岛β细胞Bcl-2/Bax表达的影响[J].兰州文理学院学报,2017,31(6):98-104.
[17]
Dohm GL. Invited review: Regulation of skeletal muscle GLUT4 expression by exercise[J]. J Appl Physiol, 2002, 93(2):782-787.
[18]
Lecturque A, Loizeau M, Vaulont S, et al. Improvement of insulinacti on in diabetic transgenic mice selectively overepressing GLUT 4 in skelet al muscle[J]. Diabetes, 1996, 45(1):23-27.
[19]
Holloszy JO. Exercise-induced increase in muscle insulin sensitivity[J]. J Appl Physiol, 2005, 99(1):338-343.
[20]
Ostergard T, Andersen JL, Nyholm B, et al. Impact of exercise training on insulin sensitivity, physical fitness, and muscle oxidative capacity in first-degree relatives of type 2 diabetic patients[J]. Am J Physiol Endocrinol Metab, 2006, 290(5):E998-1005.
[21]
Scherbaum PE, Williams S, Fogliano M, et al. A novel serum protein similar to Clq, produced exclusively in adipocytes[J]. J Biol Chem, 1995, 270(45):26746-26749.
[22]
Kennedy JW, HirshmanMF, Gervino EV, et al. Acute exercise induces GLUT4 translocation in skeletal muscle of normal human subjects and subjects with type 2 diabetes[J]. Diabetes, 1999, 48(5):1192-1197.
[23]
Bung P, Artal R. Gestational diabetes and exercise: a survey[J].Semin Perinatol, 1996, 20(4):328-333.
[24]
Sharma D, Shenoy S, Singh J. Effect of electrical stimulation on blood glucose level and lipid profile of sedentary type 2 diabetic patients[J]. Int J Diabetes Dev Ctries, 2010, 30(4):194-200.
[25]
林强,吴毅,胡永善.骨骼肌细骨骼肌细胞葡萄糖转运体4的研究进展胞葡萄糖转运体4的研究进展[J].中国康复医学杂志,2007,22(5):471-475.
[1] 曹雯佳, 刘学兵, 罗安果, 钟释敏, 邓岚, 王玉琳, 李赵欢. 超声矢量血流成像对2型糖尿病患者颈动脉壁剪切应力的研究[J/OL]. 中华医学超声杂志(电子版), 2024, 21(07): 709-717.
[2] 孟令凯, 李大勇, 王宁, 王桂明, 张炳南, 李若彤, 潘立峰. 袖状胃切除术对肥胖伴2型糖尿病大鼠的作用及机制研究[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 638-642.
[3] 李猛, 姜腊, 董磊, 吴情, 贾犇黎. 腹腔镜胃袖状切除术治疗肥胖合并2型糖尿病及脂肪胰的临床研究[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(05): 554-557.
[4] 刘盾, 潘晟. 不同入路腹腔镜袖状胃切除术用于肥胖症合并2型糖尿病的效果[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(02): 150-154.
[5] 赵帅, 王伟, 李瑞奇, 周家杰, 王道荣. 3D腹腔镜下袖状胃切除术治疗肥胖合并2型糖尿病的临床疗效及影响因素分析[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(02): 146-149.
[6] 程柏凯, 杨光. 高胰岛素-正葡萄糖钳夹技术评估慢性肾脏病患者胰岛素抵抗的研究进展[J/OL]. 中华肾病研究电子杂志, 2024, 13(06): 334-339.
[7] 陆文琪, 赵艳茹, 李焕娣, 樊欣娜, 王佳, 李萍. 2型糖尿病患者血清SMAD2和SOX6表达及其与蛋白尿的关系[J/OL]. 中华肾病研究电子杂志, 2024, 13(03): 145-151.
[8] 王璇, 娜扎开提·尼加提, 雒洋洋, 蒋升. 皮肤晚期糖基化终末产物浓度与2型糖尿病微血管并发症的相关性[J/OL]. 中华临床医师杂志(电子版), 2024, 18(05): 447-454.
[9] 王星, 陈园, 热孜万古丽·乌斯曼, 郭艳英. T2DM、Obesity、NASH、PCOS共同致病因素相关的分子机制[J/OL]. 中华临床医师杂志(电子版), 2024, 18(05): 481-490.
[10] 孙秀芹, 高美娟, 张琼阁, 吕凯敏, 王宏宇. 京西地区无心血管病史2型糖尿病中老年人群患心血管疾病的危险因素分析[J/OL]. 中华临床医师杂志(电子版), 2024, 18(03): 245-252.
[11] 何玉花, 钟欢妹, 王文惠, 沈永棋, 刘映云, 顾国威, 陈丹娜. 不同表型多囊卵巢综合征患者代谢指标及肥胖相关指标对多囊卵巢综合征合并代谢综合征人群的诊断效能分析[J/OL]. 中华临床实验室管理电子杂志, 2024, 12(04): 212-220.
[12] 张杨杨, 项楚淇, 朱满生. 肌少性肥胖与非酒精性脂肪性肝病间的关系以及研究进展[J/OL]. 中华肥胖与代谢病电子杂志, 2024, 10(04): 276-282.
[13] 吴晓明, 翟仰魁, 王娟, 张硕, 许杰, 潘从清. 男性2 型糖尿病患者空腹C 肽和定量胰岛素敏感性检测指数与血浆致动脉粥样硬化指数的相关性[J/OL]. 中华肥胖与代谢病电子杂志, 2024, 10(04): 288-294.
[14] 张梅, 陈卉, 李转霞, 王瑞, 李林娟. Metrnl和NLRP3炎症小体:糖尿病肾病的潜在诊断标志物[J/OL]. 中华肥胖与代谢病电子杂志, 2024, 10(03): 193-199.
[15] 吴子龙, 吴冰, 袁开盛, 胡瑞翔, 杨华, 王存川. 肥胖与胰腺疾病研究进展[J/OL]. 中华肥胖与代谢病电子杂志, 2024, 10(03): 213-219.
阅读次数
全文


摘要