观察不同浓度七氟烷对老龄小鼠术后谵妄行为的影响。

选择8～12月龄雌性C57BL/6J小鼠，随机分为浅麻醉组（28只）、深麻醉组（26只）和对照组（16只）。浅麻醉组和深麻醉组小鼠分别采用1.7%和2.7%浓度的七氟烷进行麻醉，并在20 min内进行剖腹手术。小鼠术后继续维持在1.7%和2.7%浓度的七氟烷条件下2 h后送回笼子里。分别在麻醉前24 h及麻醉后6、9、24 h进行行为学测试，观察不同浓度七氟烷麻醉对小鼠剖腹手术后行为学的影响。计量资料的比较采用方差分析，计数资料的比较采用χ²检验。

麻醉9 h后，深麻醉组小鼠与对照组小鼠相比，两组小鼠在旷场箱中央区域花费的时间和在Y迷宫新臂的探索次数及探索时间的差异均有统计学意义（P<0.05）。在麻醉9 h后，浅麻醉组小鼠与对照组小鼠相比，两组小鼠在Y迷宫新臂的探索次数的差异具有统计学意义（P<0.05）。在麻醉24 h后，浅麻醉组小鼠与对照组小鼠相比，两组小鼠在Y迷宫新臂的探索时间的差异具有统计学意义（P<0.01）。两组麻醉小鼠谵妄发生率的差异无统计学意义（P>0.05）。

七氟烷的麻醉深度可以影响术后谵妄的发生，深麻醉小鼠的谵妄发生率高于浅麻醉组小鼠。

To observe the effect of different concentrations of sevoflurane on postoperative delirium in aged mice.

Old mice aged 8-12 months were selected and randomly divided into three groups, which were the light anesthesia group (n=28), the deep anesthesia group (n=26), and the control group (n=16). The mice in the light and deep anesthesia groups were anesthetized with 1.7% and 2.7% sevoflurane, respectively, and underwent a simple dissection within 20 minutes. After the surgery was completed, the mice were allowed to remain under sevoflurane at 1.7% and 2.7% for 2 hours before being returned to their cages. Finally, a series of behavioral tests were used to determine the effect of dissection under sevoflurane anesthesia on the behavior of the mice (24 hours before, 6, 9, and 24 hours after anesthesia). Analysis of variance were used for comparison of measurement data, χ² test was used for comparison of counting data.

At 9 hours after anesthesia, the differences in time spent in the central area of the agora box and the number of explorations in the new arm of the Y-maze and exploration time were statistically significant in the deeply anesthetized group of mice compared with the control group (P < 0.05). At 9 hours after anesthesia, the difference in the number of explorations in the new arm of the Y-maze was statistically significant in the lightly anesthetized mice compared with the control mice (P < 0.05). At 24 hours anesthesia, the difference in the time spent in the exploration in the new arm of Y-maze was statistically significant in the lightly anesthetized mice compared with the control mice (P < 0.01). There was no significant difference in delirium incidence between the two anesthetized group (P > 0.05).

The depth of anesthesia induced by sevoflurane can affect the incidence of postoperative delirium, and the incidence of delirium was higher in the deep-anesthetized mice than in the light-anesthetized mice.