探讨红景天苷对MPTP诱导的帕金森病小鼠模型神经元及自主神经功能的保护作用。

将成年雄性C57/6J小鼠随机分为对照组（正常小鼠）、不用药的PD模型组（单纯模型组）、红景天苷治疗的PD模型组（红景天苷干预组，在MPTP给药前2周开始喂食溶解在饮用水中的红景天苷，40 mg·kg^-1·d^-1），每组6～8只。采用MPTP（10 mg/kg）腹腔注射建模，每天给药1次，连续给药4 d，在最后一针注射后的第7天处死小鼠。处死小鼠前，于麻醉状态下给予升压药和降压药，记录其血压及心率情况。处死小鼠后取待检脑组织，利用免疫组化法计数3组小鼠中脑黑质（substantia nigra pars compacta, SNpC）区、脑干孤束核（nucleus tractus solitaril, NTS）区和脑干延髓腹外侧（rostral ventrolateral medullary, RVLM）区的多巴胺神经元并比较分析。多组间的比较采用方差分析，进一步两两比较采用LSD-t检验。

3组小鼠仅SNpC区神经元计数的差异有统计学意义（F=81.920，P<0.01），其中红景天苷干预组的神经元计数明显多于单纯模型组（P<0.05）；3组小鼠RVLM区及NTS区神经元计数的差异均无统计学意义（F=7.970、11.660，均P>0.05）。3组小鼠心率变异性指标（交感神经活性、副交感神经活性以及两者比值）的差异均无统计学意义（F=0.97、1.57、1.05，均P>0.05）。3组小鼠麻醉状态下急性颈动脉插管压力反射斜率的差异均无统计学意义（F=1.500、1.940，均P>0.05）。

红景天苷对于MPTP导致的SNpC纹状体多巴胺神经元的死亡具有保护作用，可以明显抑制PD的病理进程，但对自主神经功能无保护作用。

To investigate the protective effect of salidroside on neurons and autonomic nervous function in MPTP induced Parkinson's disease mouse model.

19 adult male C57 / 6J mice were randomly divided into control group (normal mice), PD model group without medication (PD group), and PD model group treated with salidroside (salidroside intervention group, salidroside dissolved in drinking water, 40 mg·kg^-1·d^-1, 2 weeks before MPTP administration), with 6-8 mice in each group. MPTP (10 mg/kg) was injected intraperitoneally once a day for 4 days. The mice were killed on the 7th day after the last injection. Before the mice were killed, the pressor and antihypertensive drugs were given under anesthesia, and the blood pressure and heart rate were recorded. After the mice were killed, dopaminergic neurons in substantia nigra pars compacta (SNPC), nucleus tractus solitaril (NTS) and rostral ventrolateral medullary (RVLM) were counted and analyzed by immunohistochemistry. Analysis of variance was used for comparison among groups, and LSD-t test was used for further pairwise comparison.

There was significant difference in the number of neurons in SNPC area among the three groups (F=81.920, P < 0.01), and the number of neurons in salidroside intervention group was significantly higher than that in model group (P < 0.05). There was no significant difference in the number of neurons in RVLM and NTS among the three groups (F=7.970, 11.660, P > 0.05). There was no significant difference in sympathetic activity, parasympathetic activity and the ratio of the two among the three groups (F=0.97, 1.57, 1.05, P > 0.05). There was no significant difference in the slope of baroreflex among the three groups (F=1.500, 1.940, P > 0.05).

Salidroside has protective effects on the midbrain nigrostriatal dopaminergic neurons caused by MPTP, which can significantly inhibit the pathological process of PD. However, it has no protective effects on autonomic function.